Create a Planned Run for mixed samples with a Planned Run template

To plan a run for DNA and Fusion sample pairs and several individual Fusion or DNA samples, start with a DNA and Fusions Planned Run template. Then alter the template to accommodate single samples on the same chip. The following example is the procedure for creating a mixed Sample Set consisting of two sample pairs, one DNA-only sample, and two Fusion-only samples.

  1. In the Plan tab, click Templates, then click the DNA and Fusions research application.
  2. Identify a DNA and Fusions template for the instrument system that you use, then in that row click (Actions) > Copy.
  3. Enter a Template Name and select a DNA Target Regions file, then click Copy Template.
  4. Return to the Templates screen, click the DNA and Fusions research application, then select the copied template.
  5. In the Ion Reporter step of the workflow bar, select an Ion Reporter™ Software account and analysis workflow, ensure that the DNA and Fusions Sample Grouping is selected, then click Next.
  6. Ensure that the DNA and Fusions (Single Library) research application and the AmpliSeq HD DNA and Fusions (Single Library) target technique is selected, then click Next.
  7. Click Plan in the workflow bar.
  8. (Optional) The optimal number of barcodes to use with the selected panel/template is set as the default, but you can change it. If necessary, enter the number of barcodes, then click .
  9. (Optional) Clear the Same sample for DNA and Fusions option. This option is available for DNA and fusion applications.
  10. Rename the samples if desired. To rename the samples, in the Samples table, in the Sample Name column, click the sample name, then enter a new name.
  11. (Optional) Change the DNA/Fusions selections to match samples. To change the DNA/Fusions selection for a sample, navigate to the DNA/Fusions column in the Samples Table, then select DNA or Fusions in the row of the sample.
  12. (Optional) Select a Cancer Type for each sample.
  13. Select the appropriate Ion Reporter™ Software analysis workflows. To select an analysis workflow, click the existing selection in the Samples Table, then select an analysis workflow from the list.
  14. In the Samples Table, select a Relation and Gender , then click Plan Run at the bottom of the screen.

    For more information, see Create a Planned Run with Sample Sets.

Related Topics
Parent topic: Create Planned Runs
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