Test fragments

Test fragments are known genetic sequences that are used to measure the quality of your chip loading and sequencing run. You can include test fragments in your sequencing run and, after the run completes, review the Details section of the run report to evaluate the quality of your loading and sequencing run. For example, TF_1 is a single known sequence fragment that is added along with the customer library at the beginning of templating and is processed through sequencing. TF_C is already templated on ISPs and added after enrichment so goes through the loading and sequencing portions of the workflow.

Test fragments are displayed when there are at least 500 high-quality reads, with an 85% match against the appropriate template in the Test Fragment list.