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Download Barcode Summary Report
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A table listing the sample name for each barcode, total reads, aligned reads on targets, and number of targets detected.
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Download absolute read counts data
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A table listing read counts for each barcoded sample along with gene annotations.
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Download RPM data (normalized by total read counts)
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A table listing RPM (Read count per million mapped reads) for each barcoded sample along with gene annotations. RPM is calculated as:
(read count) × 106 / total number of mapped reads
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Download mean housekeeping scaled log2 RPM data
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A table listing housekeeping-gene normalized, log2-transformed read counts for each barcoded samples along with gene annotations. Conceptually, these values are read count normalized by the average expression of housekeeping (hk) genes rather than by the total number of mapped reads as described above. The values are calculated as:
These values are useful for differential analysis when a large proportion of the target genes (non-housekeeping genes) are expected to be differentially expressed or when the expression levels of the housekeeping genes in the 2 groups differ significantly.
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Download CHP files normalized by RPM
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The RPM data is converted to CHP file format for use with Transcriptome Analysis Console (TAC) software. The downloaded ZIP file contains all the CHP files from the sequencing run. Each barcoded sample has one CHP file.
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Download CHP files normalized by mean housekeeping genes
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Similar to the above CHP file, but data in these CHP files are normalized by housekeeping genes.
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Download background expression from genomic DNA and H2O neg_control
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A table containing background expression (in absolute read count) from four experiments using genomic DNA and H2O as negative control samples.
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