Run the variantCaller plugin manually

You can run the variantCaller plugin on a completed run report in Torrent Suite™ Software. If the sequencing run includes barcodes, you can apply one configuration that you saved previously to all barcodes. You can also apply a different configuration for individual barcodes. For more information, see Create a custom configuration for the variantCaller plugin.

  1. In the Data tab, click Completed Runs & Reports screen, then click the Report Name link for the completed sequencing run of interest.
  2. Click Plugins > Select Plugins to Run, then click the name of the plugin that you want to run.
  3. In the Select a Plugin to Run dialog box, select the variantCaller plugin, then click Configure.

    The Configure Plugin window opens. The variantCaller plugin parameter settings change according to the selections you make. Default settings can be different depending on the sequencer in use.

  4. Select settings that are appropriate for the sequencing instrument that is used, the experiment, and the frequency of the variants of interest. For more information, see variantCaller plugin configuration.
  5. When your changes are complete, click Submit.

    The variantCaller plugin reruns, then applies the changes that you made.