Throughput considerations

To assess a run, examine the throughput or total bases. Consider whether these numbers make sense for the application and chip type.

Chip type	Number of addressable wells	Number of reads	Throughput
Chip type	Number of addressable wells	Number of reads	200 Base Read	400 Base Read
Ion 510™ Chip	~6 million	2–3 million	0.3–0.5 Gb	0.6–1 Gb
Ion 520™ Chip	~12 million	4–6 million	0.6–1 Gb	1.2–2 Gb
Ion 530™ Chip	~37 million	15–20 million	3–4 Gb	6–8 Gb
Ion 540™ Chip	~150 million	60–80 million	10–15 Gb	N/A
Ion 550™ Chip	~260 million	100–130 million	20–25 Gb	N/A

The following table provides information regarding the number of wells per chip type and sequencing throughput specifications for sequencing runs from an Ion PGM™ System.

Chip type	Number of addressable wells	Number of reads	Throughput^[1]
Chip type	Number of addressable wells	Number of reads	200 Base Read	400 Base Read
Ion 314™ Chip v2 BC	~1.2 Million	400–500 Thousand	30–50 Mb	60–100 Mb
Ion 316™ Chip v2 BC	~6 Million	2–3 Million	300–600 Mb	600 Mb–1 Gb
Ion 318™ Chip v2 BC	~11 Million	4–5.5 Million	600 Mb–1Gb	1.2–2 Gb

¹ Expected output with >99% aligned accuracy. Output is dependent on read length and application.

The throughput, total bases, and read length metrics are found at the top of the run report. This example is from the 200-base read run (500 flows) on an Ion 540™ Chip with a CEPH control library.

Total bases or throughput depends on the ISP loading density, total reads (usable sequence), and read length.

Throughput (Total Bases)
Total Reads
Read Length

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Throughput considerations