Hotspots file format

The track line is required in a hotspots BED file.

The following is an example track line:

track name="ASD270245" description="HotSpots locations for AmpliSeq ASD270245" type=bedDetail db=hg38 reference=GRCh38.p2

The track line includes these tab-separated fields:

Field	Type	Description
`track name` (Optional)	String	A unique design identifier.
`description` (Optional)	String	A description of the design.
`type` (Required)	String	Must be `bedDetail` (without quotes).
`db` (Optional)	String	The UCSC Assembly identifier.
`reference` (This field is optional for hg19, and is required for GRCh38.)	String	The Ion Torrent™ Server reference identifier.

In hotspots BED files, the coordinates lines require these tab-separated fields:

Field	Type	Description
`chrom`	String (chars >= 0x20, other than \tab)	The name of the chromosome. This name must be an exact match with a chromosome in the reference.
`chromStart`	Unsigned int64	The starting position of the feature (zero-based).
`chromEnd`	Unsigned int64	The ending position of the feature (not inclusive). Must be greater than `chromStart`.
`HotSpotName`	String	The name is either the COSMIC identifier, dbSNP identifier, or is user-defined. If missing, the following string is used "`chrom`" + ":" + "`chromStart`" + "-" + "`chromEnd`"
`HotSpotAlleles`	String	A string that describes the variant using the following format: `REF=reference_allele` ; `OBS=observed_allele` ; `ANCHOR=base_before_allele`
`AmpliconID`	String	The Amplicon identifier. If missing, the following string is used "`chrom`" + ":" + "`chromStart`" + "-" + "`chromEnd`"