4-column target regions BED file format

The 4-column target regions BED file format is used when gene names are not known and some or all amplicon IDs are known.

The track line is optional. If present, it includes these tab-separated fields:

Field	Type	Description
`name`	String	A unique design identifier. Optional.
`description`	String	A description of the design. Optional.

The following is an example track line:

track name="ASD270245" description="AmpliSeq Pool ASD270245"

In a 4-column target regions BED file, the coordinates lines require the following tab-separated fields:

Field	Type	Description
`chrom`	String (chars >= 0x20, other than \tab)	The name of the chromosome. This name must be an exact match with a chromosome in the reference.
`chromStart`	Unsigned int64	The starting position of the feature (zero-based).
`chromEnd`	Unsigned int64	The ending position of the feature (not inclusive). Must be greater than `chromStart`.
`AmpliconID`	String	The amplicon ID. If missing, the following string is used "`chrom`" + ":" + "`chromStart`" + "-" + "`chromEnd`"

Partial example of a 4-column target regions BED file:

chr9 133738312 133738379 amplID73150 
chr9 133747484 133747542 amplID73075 
chr9 133748242 133748296 amplID73104 
chr9 133748388 133748452 491413 
chr9 133750331 133750405 74743 
chr9 133738312 133738379 73150 
chr9 133747484 133747542 73075 
chr9 133748242 133748296 73104 
chr9 133748388 133748452 491413 
chr9 133750331 133750405 74743 
chr14 105246407 105246502 329410 
chr2 29432658 29432711 34014

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4-column target regions BED file format