Long INDEL Assembler module advanced settings

The Long INDEL Assembler module advanced parameter settings control the behavior of the Long Indel Assembler module, a module within the variantCaller plugin.

IMPORTANT! The advanced parameters settings are recommended for advanced users only. If you need help setting advanced parameters, contact your local Field Service Engineer.

Parameter

Description

kmer_len

Size of the smallest k-mer used in assembly.

Impact: Increasing values make INDEL calls less sensitive but more specific.

Allowed values: Integers ≥ 5

Suggested trial value: 11 and 30

min_var_freq

Minimum frequency of the variant to be reported.

Impact: Increasing values make INDEL calls less sensitive but more specific.

Allowed values: Decimal numbers between 0 and 1

Suggested trial value: 0.1 and 0.4

min_var_count

Minimum support for a variant to be evaluated.

Impact: Increasing values make INDEL calls less sensitive but more specific.

Allowed values: Integers > 1

Suggested trial value: 3 and 30

short_suffix_match

Minimum assembled sequence match on both sides of the variant.

Impact: Increasing values make INDEL calls less sensitive but more specific.

Allowed values: Integers > 2

Suggested trial value: 4 and kmer_len

min_indel_size

Minimum size indel reported by assembly.

Impact: Increasing values make INDEL calls less sensitive but more specific.

Allowed values: Integers > 0

Suggested trial value: 2 and 30

max_hp_length

Variants containing HP larger than this are not reported.

Impact: Increasing values make INDEL calls more sensitive but less specific.

Allowed values: Integers > 1

Suggested trial value: 2 and 11

relative_strand_bias

Variants with strand bias above this are not reported.

Impact: Increasing values make INDEL calls more sensitive but less specific.

Allowed values: Decimal numbers between 0 and 1

Suggested trial value: 0.6 and 1.0

output_mnv

Enables reporting of complex variants.

Allowed values: 1 = report complex variants, 0 = don't report

Suggested trial value: 0