Well classification

Empty and loaded wells are separated by the differences in buffering over the chip during the nucleotide key flows (flows 1-8). Wells that are loaded with ISPs and associated polymerase have greater buffering capacity than empty wells and the software uses this difference to identify and classify loaded versus empty wells.

Live versus dud (non-templated ISPs) and TF versus Library ISPs are also identified based on data from the key flows. Therefore, both sequencing primer and sequencing polymerase are required for proper well classification.