8-column target regions BED file format

An 8-column target regions BED file format is for fusion panels.

In an 8-column target regions BED file, the coordinates lines require the following tab-separated fields. The format is similar to the 6-column target regions BED file, with two additional columns. The additional columns are score and strand.

Field

Type

Description

chrom

String (chars ≥ 0x20, other than \tab)

The name of the chromosome. This name must be an exact match with a chromosome in the reference.

chromStart

Unsigned int64

The starting position of the feature (zero-based).

chromEnd

Unsigned int64

The ending position of the feature (not inclusive). Must be greater than chromStart.

AmpliconID

String

Amplicon ID. If missing, the following string is used "chrom" + ":" + "chromStart" + "-" + "chromEnd"

ID

String

A customer-specified ID. If missing, set to '.'. This field is not used.

GeneSymbol

String

The gene name. If missing, set to '.'.

score

Unsigned int64

Score. If missing, set to "."

strand

String (+ or -)

Strand. If unknown, set to "+".