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Report
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Name of the analysis run report.
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Status
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Status of the analysis (for example, Started, Complete).
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Flows
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Number of flow cycles from the actual sequencing run.
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TF Name*
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Test Fragment Name.
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Q10 Mean*
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Average Q10 read length.
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Q17 Mean*
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Average Q17 read length.
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System SNR*
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System Signal-to-Noise Ratio.
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50Q10 Reads*
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Number of TF Ion Sphere™ Particles (ISP) at 50+ bp at Q10.
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50Q17 Reads*
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Number of TF Ion Sphere™ Particles (ISP) at 50+ bp at Q17.
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Keypass Reads*
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Number of reads that have test fragment keys.
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TF Key Peak Counts*
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Signal strength of the first three bases of the TF key.
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Total_Num_Reads
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Total number of reads.
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Library_50Q10_Reads
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Reads of length at least 50bp with 90% or greater accuracy.
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Library_100Q10_Reads
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Reads of length at least 100bp with 90% or greater accuracy.
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Library_200Q10_Reads
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Reads of length at least 200bp with 90% or greater accuracy.
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Library_Mean_Q10_Length
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Average length of reads with 90% or greater accuracy.
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Library_Q10_Coverage
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Average per base coverage considering reads with 90% or greater accuracy.
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Library_Q10_Longest_Alignment
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Longest read length amongst reads with 90% or greater accuracy.
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Library_Q10_Mapped Bases
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Total bases from reads with 90% or greater accuracy.
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Library_Q10_Alignments
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Number of alignments from reads with 90% or greater accuracy.
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Library_50Q17_Reads
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Reads of length at least 50bp with 98% or greater accuracy.
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Library_100Q17_Reads
|
Reads of length at least 100bp with 98% or greater accuracy.
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Library_200Q17_Reads
|
Reads of length at least 200bp with 98% or greater accuracy.
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Library_Mean_Q17_Length
|
Average length of reads with 98% or greater accuracy.
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Library_Q17_Coverage
|
Average per base coverage considering reads with 98% or greater accuracy.
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Library_Q17_Longest_Alignment
|
Longest read length amongst reads with 98% or greater accuracy.
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Library_Q17_Mapped Bases
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Total bases from reads with 98% or greater accuracy.
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Library_Q17_Alignments
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Number of alignments from reads with 98% or greater accuracy.
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Library_50Q20_Reads
|
Reads of length at least 50 bp with 99% or greater accuracy.
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Library_100Q20_Reads
|
Reads of length at least 100 bp with 99% or greater accuracy.
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Library_200Q20_Reads
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Reads of length at least 200 bp with 99% or greater accuracy.
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Library_Mean_Q20_Length
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Average length of reads with 99% or greater accuracy.
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Library_Q20_Coverage
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Average per base coverage considering reads with 99% or greater accuracy.
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Library_Q20_Longest_Alignment
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Longest read length amongst reads with 99% or greater accuracy.
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Library_Q20_Mapped_Bases
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Total bases from reads with 99% or greater accuracy.
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Library_Q20_Alignments
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Number of alignments from reads with 99% or greater accuracy.
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Library_Key_Peak_Counts
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Signal strength of the first three bases of the library key.
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Library_50Q47_Reads
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Number of perfect reads of length at least 50 bp.
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Library_100Q47_Reads
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Number of perfect reads of length at least 100 bp.
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Library_200Q47_Reads
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Number of perfect reads of length at least 200 bp.
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Library_Mean_Q47_Length
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Average length of perfect reads.
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Library_Q47_Coverage
|
Average per-base coverage considering only perfect reads.
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Library_Q47_Longest_Alignment
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Longest reads length amongst perfect reads.
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Library_Q47_Mapped_Bases
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Total bases from perfect reads.
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Library_Q47_Alignments
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Number of alignments from perfect reads.
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Library_CF
|
CAFIE metric: Carry forward.
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Library_IE
|
CAFIE metric: Incomplete extension.
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Library_DR
|
CAFIE metric: Signal/polymerase loss (droop).
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Library_SNR
|
System signal-to-noise ratio.
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Sample
|
Name of the sample.
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Library
|
Name of the reference genome.
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Notes
|
Any additional user-provided notes.
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Run Name
|
Long name of the analysis run.
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PGM Name
|
Name of the Ion PGM™ or Ion Proton™ instrument where the sample was sequenced.
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Run Date
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Date that the sample was sequenced.
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Run Directory
|
Location of the raw DAT files on the Ion Torrent™ Server.
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Num_Washouts
|
NA
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Num_Dud_Washouts
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NA
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Num_Washout_Ambigous
|
NA
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Num_Washout_Live
|
NA
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Num_Washout_Test_Fragment
|
NA
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Num_Washout_Library
|
NA
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Library_Pass_Basecalling
|
NA
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Library_pass_Cafie
|
NA
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Number_Ambiguous
|
NA
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Number_Live
|
Number of wells producing a signal.
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Number_Dud
|
Number of wells with ISPs but no signal.
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Number_TF
|
Number of wells containing test fragment.
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Number_Lib
|
Number of wells containing library.
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Number_Bead
|
Number of wells containing beads.
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Library_Live
|
Number of wells containing library ISP with signal.
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Library_Keypass
|
Number of wells containing library ISP with signal and match key.
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TF_Live
|
Number of wells containing test fragment ISP with signal.
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TF_Keypass
|
Number of wells containing test fragment ISP with signal and match key.
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Keypass_All_Beads
|
Number of wells containing ISP with signal and match key.
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P
|
JSON string of plugin data.
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s
|
JSON string of plugin data.
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