DNA barcode set CSV file setup

Each row in the CSV file contains information for an individual barcode in the barcode set.

Column name

Type

Description

index

Integer

A unique index for an individual barcode (for example, 1, 2, 3,...)

Only numerical characters are accepted.

id_str

String

A unique name for an individual barcode entry. Typically the name of the barcode kit followed by a 3′– to 4′– digit unique identifier, such as IonXpress_001 or IonCode_0101.

sequence

(Required)

String

The 5′– barcode sequence.

Upper‑case G, C, A, and T are allowed.

adapter

String

The portion of the 5′– barcode adapter not used to identify this barcode. Often referred to as the "stuffer sequence".

Upper‑case G, C, A, and T are allowed.

annotation

--

Use for any barcode-specific notes.

end_sequence

String

The 3′– barcode sequence.

IMPORTANT! This sequence is required for libraries that were prepared using dual barcode technology.

Upper‑case G, C, A, and T are allowed.

end_adapter

String

The portion of the 3′– barcode adapter not used to identify this barcode. Often referred to as the "stuffer sequence".

This sequence is available only for libraries that were prepared using dual barcode technology.

Upper‑case G, C, A, and T are allowed.