Advanced Settings—Kits step in workflow bar

You can customize the kit advanced settings in the Kits step in the workflow bar.

IMPORTANT! We recommend using the default settings. Consult your local Field Service Engineer before modifying advanced settings.

In the Kits step in the workflow bar, expand the Advanced Settings box, then select Customize.

Edit one of the following settings.

Setting	Description
Templating Protocol	Script that the Ion Chef™ Instrument follows to perform the templating reaction. We recommend that you do NOT change this setting.
Forward Library Key	Select your forward library key, if you have one.
Test Fragment Key	Enter your test fragment key.
Base Calibration Mode	Select one of the following base calibration options: Default Calibration—allows a random subset of wells to be used for base calibration. This option uses TMAP to align the training subset of wells and is recommended if a good reference for the template is available. Enable Calibration Standard—allows wells belonging to the Calibration Standard to be selected as the training subset. Blind Calibration—uses the same random subset of wells as Default Calibration but does not require an alignment step to generate the calibration model. This option is recommended if the template does not align well to a reference genome or if no reference is specified. No Calibration See your template kit user guide for more details.
Forward 3' Adapter	Select your forward 3' adapter.
Flow Order	Select the flow order. For more information, see flow order.