Dataflow using the 400-bp kit on the Ion PGM™ Sequencer
The following table shows a high-level view of the dataflow using the 400-bp kit on the Ion PGM™ Sequencer. File sizes for Ion Torrent™ data depend on the Torrent Suite™ Software version, chip type, and kit type.
|
Step |
Resulting file type |
|||
|---|---|---|---|---|
|
Flows |
— |
900 |
900 |
900 |
|
Raw image acquisition |
DAT |
396 GB |
246 GB |
52 GB |
|
Image processing |
WELLS |
31.4 GB |
18.4 GB |
3.5 GB |
|
Signal processing and base calling |
BAM |
6.8 GB |
4.5 GB |
0.65 GB |
CAUTION! File sizes vary depending on the number of flows, the number of wells generating signals, and the number of library reads available. Your file sizes may be different. An unmapped BAM file format is used in the analysis pipeline steps before alignment.